生物催化宿主细胞表型的改造

本研究通过菌株驯化、全局转录机制工程等手段获得有具有较高机溶剂耐受性的微生物菌株，结合转录组、蛋白组等分析方法揭示以大肠杆菌为代表的微生物菌株的有机溶剂耐受性机制，为构建具有高有机溶剂耐受性的宿主菌提供有效策略，因此对生物能源和生物催化等应用领域具有重要意义。

Engineering of microbial organic-solvent-tolerance

Organic-solvent tolerant (OST) microorganisms were developed using strain adaptation and global transcription machinery engineering (gTME) approaches. DNA microarrays and proteomics were employed to assess the OST mechanisms of E. coli strains. Our results could serve as a practical strategy for the construction of platform E. coli strains for applications in biofuel production and biocatalysis.

项目资助：

国家自然科学基金面上项目，21276112